Biochemical Characterization of Agaricus bisporus Dikaryon Strains
نویسندگان
چکیده
منابع مشابه
Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus)
To promote the selection of promising monokaryotic strains of button mushroom (Agaricus bisporus) during breeding, 61 progeny strains derived from basidiospores of two different lines of dikaryotic parental strains, ASI1038 and ASI1346, were analyzed by nucleotide sequencing of the intergenic spacer I (IGS I) region in their rDNA and by extracellular enzyme assays. Nineteen different sizes of I...
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One of the main problems for conservation of mushroom germplasm is that the traditional method of subculturing facilitates aging and contamination of cultures. The conservation of strains at ultra-low temperature in liquid nitrogen (-196 °C) is a widely used method, however, this method become expensive in collections with large number of strains and requires highly specialized personnel. The a...
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Agrobacterium tumefaciens is known to transfer parts of its tumor-inducing plasmid to filamentous fungi including Agaricus bisporus. We have used this transformation system to transform germinating basidiospores of A. bisporus. Analysis of hygromycin B-resistant clones showed that the T-DNA integrated at random sites and in multiple copies into the host genome. Furthermore we have been able to ...
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Partially purified virus preparations from sporophores of Agaricus bisporus affected with LaFrance disease had up to a 15-fold-higher RNA-dependent RNA polymerase activity than did comparable preparations from healthy sporophores. Enzyme activity was dependent upon the presence of Mg2+ and the four nucleoside triphosphates and was insensitive to actinomycin D, a-amanitin, and rifampin. The 3H-l...
متن کاملSelected wild strains of Agaricus bisporus produce high yields of mushrooms at 25°C.
BACKGROUND To cultivate the button mushroom Agaricus bisporus in warm countries or during summer in temperate countries, while saving energy, is a challenge that could be addressed by using the biological diversity of the species. AIMS The objective was to evaluate the yield potential of eight wild strains previously selected in small scale experiments for their ability to produce mature frui...
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ژورنال
عنوان ژورنال: The Korean Journal of Mycology
سال: 2014
ISSN: 0253-651X
DOI: 10.4489/kjm.2014.42.1.86